Microbial polysaccharides represent a class of important products of growing interest for many sectors of industry. Exopolysaccharides epss are oriental journal of chemistry. This research study gives a wide range of discovery on novel exopolysaccharides producing bacteria strains. Exopolysaccharides and lipopolysaccharide production by. Marine bacterial exopolysaccharides epss from extreme. Polysaccharides are industrially used as thickeners, stabilisers and gelling agents in food products. Here we report methods to extract and purify high molecular weight 15 kda exopolysaccharides from biofilms of eight human pathogens, including species of staphylcococcus. Isolation and screening of exopolysaccharide producing bacterial eps from potato rhizosphere for soil aggregation muminah1, baharuddin2, hazarin subair3, fahruddin4 and baso darwisah5 1graduate school, hasanuddin university, makassar 90245, indonesia 1department of plantation crop, agriculture polytechnic pangkep, pangkep 90655, indonesia. Microbial exopolysaccharides have a wide range of applications depending on their nature, composition, and structure. In presence of mannitol and sucrose higher eps production was observed in all mutant strains. For the 27 bacterial strains evaluated, small quantities of eps inhibited cell.
The book takes an interdisciplinary view that examines this fascinating subject area in detail from molecular biology, genome, transcriptome and proteomewide perspectives, and looks at the ecological. Isolation and screening of exopolysaccharide producing. Exopolysaccharides epss produced by lactic acid bacteria lab are a topic of research for scientists due to their technological application in the dairy industry where they improve the viscosity, texture and mouthfeel of yoghurt and fermented milks and act as fat substitutes in cheesemaking. The ameba, the ciliates, coccidia, and microsporidia, the flagellates, the cestodes tapeworms, the nematodes roundworms, the trematodes flukes, unusual tissue dwelling nematodes, larval cestodes and nematodes which infect man, malaria, the. Aboelwafa2 1water pollution research department, national research centre, dokki, giza, egypt, 12622. Many new marine microbial epss with novel chemical compositions.
In this chapter, data on eps production and the effect of eps on corrosion of steel produced by lactobacillus sp. The soil samples were taken from three different land of slopes at elevation 1500 m above sea level at malino,south sulawesi. Significantly, increased production was noticed in case of mutants r02 0521. Prajapati2 1senior officer, banskantha district cooperative milk producers union ltd, banas dairy, palanpur, gujarat. Microbial exopolysaccharide an inevitable product for. Microbial polysaccharides are produced in two forms, capsular polysaccharide cps and exopolysaccharide eps. The material properties of exopolysaccharides have revolutionized the industrial and medical sectors due to their retinue of functional applications and prospects. Biochemistry and functional analysis of exopolysaccharide production in lactobacillus johnsonii abstract lactic acid bacteria lab produce unique exopolysaccharides eps that are important in food industry but they also play critical role in bacterial interactions during colonisation of the gastrointestinal tract. The physiological role of eps depends on the ecological niches and the natural environment in which microorganisms have been isolated. Exopolysaccharides produced by lactic acid bacteria of. Monosaccharide units composition, linkages, charge and size determine the eps. Morphologic and functional properties of bacterial exopolysaccharide. Generally a limited number of monosaccharides comprise eps. Although this description does not take into account groups of free floating microbial aggregates flocs.
Citeseerx document details isaac councill, lee giles, pradeep teregowda. Some bacterial epss can directly replace polysaccharides extracted from plants e. Production and characterization of exopolysaccharide. It is clear that microorganisms undergo profound changes during their transition from planktonic free swimming organisms to cells that are part of a complex. The health benefits that are continually being associated with these polysaccharides enable the development of dual function, addedvalue, and cleanlabel products. Receive free email alerts when new articles cite this article. Evaluation of exopolysaccharides eps and nonexopolysaccharides non eps producing strains of lactic acid bacteria to select the best combinations of cultures for dahi preparation geeta v. Rhizobial plant symbionts and bacterial plant pathogens produce exopolysaccharides that. Exopolysaccharides producing bacteria strains screening. More recently they were used as depollution agents and there was a growing interest in their biological functions like antitumor, antioxidant or prebiotic activities liu et al. The genus leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides eps. Ability of microorganisms to produce eps is direct and logical response to selective pressures in that natural environment.
Diverse structural, functional and valuable polysaccharides are synthesized by bacteria of all taxa and secreted into the external environment. Abstract biofilms can be defined as communities of microorganisms attached to a surface. Chemically, eps are rich in high molecular weight polysaccharides 10 to 30 kda and have heteropolymeric composition. Oantigen, and extracellular bacterial polysaccharides for.
Pdf bacterial exopolysaccharidesa perception researchgate. Diagnosing medical parasites a public health officers guide to assisting laboratory and medical officers. Exopolysaccharides are studied from hydrothermal vent bacteria, halobacteria, methanogens, autotrophic organisms, acidophiles as well as from ground water and sewage sludge microorganisms. The morphology and molecular features of exopolysaccharides also will be identified and characterized in this research. Pdf microbial polysaccharides are multifunctional and can be divided into intracellular polysaccharides, structural polysaccharides and.
Exopolysaccharides an overview sciencedirect topics. Recent advances in exopolysaccharides from paenibacillus spp production, isolation, structure, and bioactivities. Exopolysaccharides from marine bacteria springerlink. Author links open overlay panel philippe duboc beat mollet.
Applications of exopolysaccharides in the dairy industry. Synthesis, production, and biotechnological applications. Epss of microbial origin are ubiquitous in nature, have unique properties, and can be isolated from the bacteria in fresh water, marine. Systems biology of microbial exopolysaccharides production core. Characterization of exopolysaccharides produced by. Rhizobiaceae are a very diverse family of symbiotic gramnegative bacteria occurring in the soil as freeliving microorganisms or existing in. Purification and characterization of biofilmassociated.
Bacterial exopolysaccharides from extreme marine habitats. Exopolysaccharides from bacteria with novel application. Bacterial exopolysaccharides from extreme marine habitats ncbi. Exopolysaccharides it has been suggested by some research work, that a potential controlling factor in eps biosynthesis is the availability of sugar nucleotides which is necessary for construction of the polymers boels et al. Components most commonly found in marine eps are monosaccharide such as pentoses as darabinose, dribose, dxylose, hexoses dglucose, dgalactose, dmannose. Bacterial exopolysaccharides as new natural coagulants for. Biochemistry and functional analysis of exopolysaccharide. Lactobacillus plantarum ts was obtained from the collection of department of biology, shumen university. The precipitate formed was recovered by centrifuging, then dried at. Marine bacterial exopolysaccharides epss from extreme environments and their biotechnological applications prashakha j. Microbial exopolysaccharides epss synthesized by lactic acid bacteria lab play a major role in the manufacturing of fermented dairy products. To investigate the regulation mechanism of eps biosynthesis and to improve eps production through cofactor engineering, a h 2 oforming nadh oxidase gene was cloned from streptococcus mutans and overexpressed in l. Role of cyanobacterial exopolysaccharides in phototrophic biofilms and in complex. Structure and production of eps by marine bacteria.
Dave department of life sciences, maharaja krishnakumarsinhji bhavnagar university, bhavnagar364001 gujarat, india received june 24, 2016, accepted december 15, 2016 abstract. Conversely, other bacterial epss possess unique properties that can launch a range of new commercial opportunities e. Traditionally, lactic acid bacteria exopolysaccharides have an important role in fermented dairy products and more recently are being applied for the improvement of bakery products. In bacterial biofilms, high molecular weight, secreted exopolysaccharides can serve as a scaffold to which additional carbohydrates, proteins, lipids, and nucleic acids adhere, forming the matrix of the developing biofilm. Bacterial exopolysaccharides protection from intestinal inflammation. Exopolysaccharides produced by lactic acid bacteria of kefir grains ginka i. Characterization of exopolysaccharides produced by thermophilic lactic acid bacteria isolated from tropical fruits of thailand. Among these, the microorganisms belonging to the archaea domain are of significant biotechnological importance as their biopolymers possess unique. Many marine bacteria produce exopolysaccharides eps as a. Download fulltext pdf potentials of exopolysaccharides from lactic acid bacteria article pdf available in indian journal of microbiology 521. These polysaccharides are referred to as exopolysaccharides and they may either be homopolymeric or heteropolymeric in composition and of diverse high molecular weights 10 to kda. Bacterial exopolysaccharides as new natural coagulants for surface water treatment raed s. Epss establish the functional and structural integrity of biofilms, and are considered the fundamental component that determines the physiochemical properties of a biofilm epss are mostly composed of polysaccharides exopolysaccharides and proteins, but. Bacteria produce a wide range of exopolysaccharides which are synthesized via different biosynthesis pathways.
This bacteria primarily affects areas containing oil glands including the face, back and. Exopolysaccharide definition of exopolysaccharide by the free dictionary. Microbial derived epss have advantages over traditionally used polysaccharides from plant source and seaweedderived gums, the later are easily affected by. Because of the wide variety of industrial applications of eps, this study aimed to produce and characterize the native exopolysaccharide. An overview of the functionality of exopolysaccharides. Polysaccharides produced by microbes can be generally classified by their biological functions into intracellular storage polysaccharides glycogen, capsular polysaccharides which are closely linked to the cell surface e. Schematic representation of the possible healthpromoting properties of exopolysaccharides produced by lactic acid bacteria. In recent years, there has been a growing interest in isolating new exopolysaccharides epssproducing bacteria from marine environments, particularly from various extreme marine environments. The bacterial exopolysaccharides represent a wide range of chemical structures, but have not yet acquired appreciable significance. Due to the extensive applications of bacterial exopolysaccharides, this overview provides basic information on their. Exploiting exopolysaccharides from lactic acid bacteria. Most epss produced by marine bacteria are heteropolysaccharides containing three or four different monosaccharides arranged in groups of 10 or less to form repeating units. Isolation and screening bacterial exopolysaccharide eps. The genes responsible for synthesis are often clustered within the genome of the respective production organism.
Exopolysaccharides eps obtained from the culture medium of lactobacillus confusus tistr 1498 were investigated to determine their molecular characteristics and the effect of molecular weight mw on immunomodulatory activity. Many marine bacteria produce exopolysaccharides eps as a strategy. Simovb a laboratory of applied microbiology, institute of microbiology, bulgarian academy of sci ences, 26 maritza blvd. Extracellular polymeric substances epss are natural polymers of high molecular weight secreted by microorganisms into their environment. Several pathophysiological associations have been identified in which propionibacterium acnes plays a major role. Biotechnology of microbial exopolysaccharides by ian w.
Extreme environments, generally characterized by atypical temperatures, ph, pressure, salinity, toxicity, and radiation levels, are inhabited by various microorganisms specifically adapted to these particular conditions, called extremophiles. Lactobacillus casei lc2w is an exopolysaccharide epsproducing strain with probiotic effects. Acne vulgaris acne is a chronic inflammatory disease prevalent among adolescents and adults, with significant psychological effects. Pdf potentials of exopolysaccharides from lactic acid. Lactic acid bacteria exopolysaccharides in foods and. Eps production is characterized by a large variety in terms of quantity, chemical composition, molecular size, charge, type of sidechains and rigidity of the molecules. Study on the antibacterial activity of exopolysaccharides. Full texthtml download pdf 737k download meta ris compatible with endnote, reference manager, procite, refworks bib tex. These polysaccharides are referred to as exopolysaccharides and they may either be homopolymeric or heteropolymeric in composition and of diverse high molecular. Bacterial exopolysaccharides as a modern biotechnological tool for. This study aimed to determine the ability of bacterial isolated from rhizosphere of potato plants in producing exopolysaccharide and growth stimulating substances such as indole acetic acid iaa.
465 105 994 933 733 1550 444 213 635 619 230 868 1246 559 197 1029 1391 1168 1162 238 536 1149 681 776 356 314 56 214 331 1239 1008 610 510 569 1236 894 681 905 702 923